Dataset Information

Development of yeast and microalgae consortium biofilm growth system for biofuel production.

ABSTRACT:

Background

The current study aimed to develop a laboratory-scale biofilm photobioreactor system for biofuel production.

Scope & approach

During the investigation, Jute was discovered to be the best, cheap, hairy, open-pored supporting material for biofilm formation. Microalgae & yeast consortium was used in this study for biofilm formation.

Conclusion

The study identified microalgae and yeast consortium as a promising choice and ideal partners for biofilm formation with the highest biomass yield (47.63 ± 0.93 g/m²), biomass productivity (4.39 ± 0.29 to 7.77 ± 0.05 g/m²/day) and lipid content (36%) over 28 days cultivation period, resulting in a more sustainable and environmentally benign fuel that could become a reality in the near future.

SUBMITTER: Bisht B

PROVIDER: S-EPMC10472003 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature

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Publications

Development of yeast and microalgae consortium biofilm growth system for biofuel production.

Bisht Bhawna B Verma Monu M Sharma Rohit R Chauhan P K PK Pant Kumud K Kim Hyunook H Vlaskin Mikhail S MS Kumar Vinod V

Heliyon 20230825 9

<h4>Background</h4>The current study aimed to develop a laboratory-scale biofilm photobioreactor system for biofuel production.<h4>Scope & approach</h4>During the investigation, Jute was discovered to be the best, cheap, hairy, open-pored supporting material for biofilm formation. Microalgae & yeast consortium was used in this study for biofilm formation.<h4>Conclusion</h4>The study identified microalgae and yeast consortium as a promising choice and ideal partners for biofilm formation with the ...[more]

PMID: 37662773

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Project description:Yarrowia lipolytica is a non-pathogenic, dimorphic and strictly aerobic yeast species. Owing to its distinctive physiological features and metabolic characteristics, this unconventional yeast is not only a good model for the study of the fundamental nature of fungal differentiation but is also a promising microbial platform for biochemical production and various biotechnological applications, which require extensive genetic manipulations. However, genetic manipulations of Y. lipolytica have been limited due to the lack of an efficient and stable genetic transformation system as well as very high rates of non-homologous recombination that can be mainly attributed to the KU70 gene. Here, we report an easy and rapid protocol for the efficient genetic transformation and for gene deletion in Y. lipolytica Po1g. First, a protocol for the efficient transformation of exogenous DNA into Y. lipolytica Po1g was established. Second, to achieve the enhanced double-crossover homologous recombination rate for further deletion of target genes, the KU70 gene was deleted by transforming a disruption cassette carrying 1 kb homology arms. Third, to demonstrate the enhanced gene deletion efficiency after deletion of the KU70 gene, we individually deleted 11 target genes encoding alcohol dehydrogenase and alcohol oxidase using the same procedures on the KU70 knockout platform strain. It was observed that the rate of precise homologous recombination increased substantially from less than 0.5% for deletion of the KU70 gene in Po1g to 33%-71% for the single gene deletion of the 11 target genes in Po1g KU70Δ. A replicative plasmid carrying the hygromycin B resistance marker and the Cre/LoxP system was constructed, and the selection marker gene in the yeast knockout strains was eventually removed by expression of Cre recombinase to facilitate multiple rounds of targeted genetic manipulations. The resulting single-gene deletion mutants have potential applications in biofuel and biochemical production.

Dataset Information

Development of yeast and microalgae consortium biofilm growth system for biofuel production.

Background

Scope & approach

Conclusion

Publications

Development of yeast and microalgae consortium biofilm growth system for biofuel production.

Similar Datasets

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