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ACD15, ACD21 and SLN regulate accumulation and mobility of MBD6 to silence genes and transposable elements.


ABSTRACT: DNA methylation mediates silencing of transposable elements and genes in part via recruitment of the Arabidopsis MBD5/6 complex, which contains the methyl-CpG-binding domain (MBD) proteins MBD5 and MBD6, and the J-domain containing protein SILENZIO (SLN). Here we characterize two additional complex members: α-crystalline domain containing proteins ACD15 and ACD21. We show that they are necessary for gene silencing, bridge SLN to the complex, and promote higher order multimerization of MBD5/6 complexes within heterochromatin. These complexes are also highly dynamic, with the mobility of complex components regulated by the activity of SLN. Using a dCas9 system, we demonstrate that tethering the ACDs to an ectopic site outside of heterochromatin can drive massive accumulation of MBD5/6 complexes into large nuclear bodies. These results demonstrate that ACD15 and ACD21 are critical components of gene silencing complexes that act to drive the formation of higher order, dynamic assemblies.

SUBMITTER: Boone BA 

PROVIDER: S-EPMC10473691 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature

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ACD15, ACD21 and SLN regulate accumulation and mobility of MBD6 to silence genes and transposable elements.

Boone Brandon A BA   Ichino Lucia L   Wang Shuya S   Gardiner Jason J   Yun Jaewon J   Jami-Alahmadi Yasaman Y   Sha Jihui J   Mendoza Cristy P CP   Steelman Bailey J BJ   van Aardenne Aliya A   Kira-Lucas Sophia S   Trentchev Isabelle I   Wohlschlegel James A JA   Jacobsen Steven E SE  

bioRxiv : the preprint server for biology 20231006


DNA methylation mediates silencing of transposable elements and genes in part via recruitment of the Arabidopsis MBD5/6 complex, which contains the methyl-CpG-binding domain (MBD) proteins MBD5 and MBD6, and the J-domain containing protein SILENZIO (SLN). Here we characterize two additional complex members: α-crystalline domain containing proteins ACD15 and ACD21. We show that they are necessary for gene silencing, bridge SLN to the complex, and promote higher order multimerization of MBD5/6 com  ...[more]

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