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A New Fluorescent Probe for Hydrogen Sulfide Detection in Solution and Living Cells.

ABSTRACT: Since Hydrogen Sulfide (H₂S) was recognized as a gas transmitter, its detection and quantification have become a hot research topic among chemists and biologists. In this area, fluorescent probes have shown great advantages: fast and strong response, low detection limit and easy manipulation. Here we developed a new fluorescent probe that detected H₂S selectively among various bioactive and inorganic salts. This probe was based on the core structure of fluorescein and reacted with H₂S through azide-reduction. Great linearity was achieved correlating fluorescence intensity and H₂S concentrations in solution. The detection of H₂S in cancer cells was also achieved.

SUBMITTER: Feng W

PROVIDER: S-EPMC10488361 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature

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A New Fluorescent Probe for Hydrogen Sulfide Detection in Solution and Living Cells.

Feng Wei W Xiao Qicai Q Wang Lu L Yang Yuanyong Y

Molecules (Basel, Switzerland) 20230823 17

Since Hydrogen Sulfide (H<sub>2</sub>S) was recognized as a gas transmitter, its detection and quantification have become a hot research topic among chemists and biologists. In this area, fluorescent probes have shown great advantages: fast and strong response, low detection limit and easy manipulation. Here we developed a new fluorescent probe that detected H<sub>2</sub>S selectively among various bioactive and inorganic salts. This probe was based on the core structure of fluorescein and react ...[more]

PMID: 37687024

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