A computer vision based optical method for measuring fluid level in cell culture plates.
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ABSTRACT:
SUBMITTER: Baudin PV
PROVIDER: S-EPMC10490923 | biostudies-literature | 2023
REPOSITORIES: biostudies-literature
Publications
A computer vision based optical method for measuring fluid level in cell culture plates.
Baudin Pierre V PV Teodorescu Mircea M
PloS one 20230908 9
For a transparent well with a known volume capacity, changes in fluid level result in predictable changes in magnification of an overhead light source. For a given well size and fluid, the relationship between volume and magnification can be calculated if the fluid's index of refraction is known or in a naive fashion with a calibration procedure. Light source magnification can be measured through a camera and processed using computer vision contour analysis with OpenCV. This principle was applie ...[more]
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