An easy adjustment of instrument settings ('Peak MALDI') improves identification of organisms by MALDI-ToF mass spectrometry.
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ABSTRACT:
SUBMITTER: Nellessen CM
PROVIDER: S-EPMC10497524 | biostudies-literature | 2023 Sep
REPOSITORIES: biostudies-literature
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An easy adjustment of instrument settings ('Peak MALDI') improves identification of organisms by MALDI-ToF mass spectrometry.
Nellessen Christopher M CM Nehl David B DB
Scientific reports 20230912 1
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-ToF MS) is a mature technolaogy with 'auto-execute' instrument settings and peak processing parameters tailored for rapid bacterial identification. Adoption for other organisms has been problematic, with optimisation efforts focusing on sample preparation. Using the Bruker MALDI Biotyper, we demonstrate 'Peak MALDI': easily-applied settings that immediately enhance sensitivity, improve spectrum quality, and incre ...[more]
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