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CRISPR-based engineering of RNA viruses.

ABSTRACT: CRISPR RNA-guided endonucleases have enabled precise editing of DNA. However, options for editing RNA remain limited. Here, we combine sequence-specific RNA cleavage by CRISPR ribonucleases with programmable RNA repair to make precise deletions and insertions in RNA. This work establishes a recombinant RNA technology with immediate applications for the facile engineering of RNA viruses.

SUBMITTER: Nemudryi A

PROVIDER: S-EPMC10499312 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature

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CRISPR-based engineering of RNA viruses.

Nemudryi Artem A Nemudraia Anna A Nichols Joseph E JE Scherffius Andrew M AM Zahl Trevor T Wiedenheft Blake B

Science advances 20230913 37

CRISPR RNA-guided endonucleases have enabled precise editing of DNA. However, options for editing RNA remain limited. Here, we combine sequence-specific RNA cleavage by CRISPR ribonucleases with programmable RNA repair to make precise deletions and insertions in RNA. This work establishes a recombinant RNA technology with immediate applications for the facile engineering of RNA viruses. ...[more]

PMID: 37703376

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CRISPR-based engineering of RNA viruses.

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