Purification of Encephalitozoon cultures contaminated by mycoplasmas by murine intraperitoneal inoculation.
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ABSTRACT:
SUBMITTER: Ridoux O
PROVIDER: S-EPMC105057 | biostudies-literature | 1998 Aug
REPOSITORIES: biostudies-literature
Publications
Purification of Encephalitozoon cultures contaminated by mycoplasmas by murine intraperitoneal inoculation.
Ridoux O O Foucault C C Drancourt M M
Journal of clinical microbiology 19980801 8
Encephalitozoon species are strict intracellular microsporidia. Cocultures with eukaryotic cell lines can become accidently contaminated by mycoplasmas. We propose a decontamination protocol based on differential cell targeting after intraperitoneal inoculation in mice. Mycoplasma-free microsporidia were isolated from the brains and spleens of inoculated mice 24 h postinoculation by using the centrifugation shell vial system. Identification was confirmed by direct sequencing of PCR-amplified 16S ...[more]
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