Purification of Encephalitozoon cultures contaminated by mycoplasmas by murine intraperitoneal inoculation.

ABSTRACT: Encephalitozoon species are strict intracellular microsporidia. Cocultures with eukaryotic cell lines can become accidently contaminated by mycoplasmas. We propose a decontamination protocol based on differential cell targeting after intraperitoneal inoculation in mice. Mycoplasma-free microsporidia were isolated from the brains and spleens of inoculated mice 24 h postinoculation by using the centrifugation shell vial system. Identification was confirmed by direct sequencing of PCR-amplified 16S rRNA.

SUBMITTER: Ridoux O 

PROVIDER: S-EPMC105057 | biostudies-literature | 1998 Aug

REPOSITORIES: biostudies-literature