Development of specific nested oligonucleotide PCR primers for the Streptococcus iniae 16S-23S ribosomal DNA intergenic spacer.
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ABSTRACT:
SUBMITTER: Berridge BR
PROVIDER: S-EPMC105208 | biostudies-literature | 1998 Sep
REPOSITORIES: biostudies-literature
Publications
Development of specific nested oligonucleotide PCR primers for the Streptococcus iniae 16S-23S ribosomal DNA intergenic spacer.
Berridge B R BR Fuller J D JD de Azavedo J J Low D E DE Bercovier H H Frelier P F PF
Journal of clinical microbiology 19980901 9
Streptococcus iniae is a cause of septicemia, meningoencephalitis, and death in farmed fish and of cellulitis in human beings. A set of nested oligonucleotide PCR primers that specifically amplified a 373-bp subunit from a variety of clinical isolates from farmed fish and human patients were constructed from a 524-bp consensus sequence of the S. iniae 16S-23S ribosomal DNA intergenic spacer. ...[more]
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