Unknown

Dataset Information

0

Tracking B cell responses to the SARS-CoV-2 mRNA-1273 vaccine.


ABSTRACT: Protective immunity following vaccination is sustained by long-lived antibody-secreting cells and resting memory B cells (MBCs). Responses to two-dose SARS-CoV-2 mRNA-1273 vaccination are evaluated longitudinally by multimodal single-cell analysis in three infection-naïve individuals. Integrated surface protein, transcriptomics, and B cell receptor (BCR) repertoire analysis of sorted plasmablasts and spike+ (S-2P+) and S-2P- B cells reveal clonal expansion and accumulating mutations among S-2P+ cells. These cells are enriched in a cluster of immunoglobulin G-expressing MBCs and evolve along a bifurcated trajectory rooted in CXCR3+ MBCs. One branch leads to CD11c+ atypical MBCs while the other develops from CD71+ activated precursors to resting MBCs, the dominant population at month 6. Among 12 evolving S-2P+ clones, several are populated with plasmablasts at early timepoints as well as CD71+ activated and resting MBCs at later timepoints, and display intra- and/or inter-cohort BCR convergence. These relationships suggest a coordinated and predictable evolution of SARS-CoV-2 vaccine-generated MBCs.

SUBMITTER: Lopes de Assis F 

PROVIDER: S-EPMC10529190 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature

altmetric image

Publications


Protective immunity following vaccination is sustained by long-lived antibody-secreting cells and resting memory B cells (MBCs). Responses to two-dose SARS-CoV-2 mRNA-1273 vaccination are evaluated longitudinally by multimodal single-cell analysis in three infection-naïve individuals. Integrated surface protein, transcriptomics, and B cell receptor (BCR) repertoire analysis of sorted plasmablasts and spike<sup>+</sup> (S-2P<sup>+</sup>) and S-2P<sup>-</sup> B cells reveal clonal expansion and ac  ...[more]

Similar Datasets

2023-07-07 | GSE219098 | GEO
| PRJNA907025 | ENA
| S-EPMC8385554 | biostudies-literature
| S-EPMC7727324 | biostudies-literature
| S-EPMC7787219 | biostudies-literature
| S-EPMC7944952 | biostudies-literature
| S-EPMC8691522 | biostudies-literature
| S-EPMC8809505 | biostudies-literature
| S-EPMC8601286 | biostudies-literature
| S-EPMC7449230 | biostudies-literature