Dataset Information

Proteomic profiling identifies novel inflammation-related plasma proteins associated with ischemic stroke outcome.

ABSTRACT:

Background

The inflammatory response to cerebral ischemia is complex; however, most clinical studies of stroke outcome focus on a few selected proteins. We, therefore, aimed to profile a broad range of inflammation-related proteins to: identify proteins associated with ischemic stroke outcome that are independent of established clinical predictors; identify proteins subsets for outcome prediction; and perform sex and etiological subtype stratified analyses.

Methods

Acute-phase plasma levels of 65 inflammation-related proteins were measured in 534 ischemic stroke cases. Logistic regression was used to estimate associations to unfavorable 3-month functional outcome (modified Rankin Scale score > 2) and LASSO regressions to identify proteins with independent effects.

Results

Twenty proteins were associated with outcome in univariable models after correction for multiple testing (FDR < 0.05), and for 5 the association was independent of clinical variables, including stroke severity (TNFSF14 [LIGHT], OSM, SIRT2, STAMBP, and 4E-BP1). LASSO identified 9 proteins that could best separate favorable and unfavorable outcome with a predicted diagnostic accuracy (AUC) of 0.81; three associated with favorable (CCL25, TRAIL [TNFSF10], and Flt3L) and 6 with unfavorable outcome (CSF-1, EN-RAGE [S100A12], HGF, IL-6, OSM, and TNFSF14). Finally, we identified sex- and etiologic subtype-specific associations with the best discriminative ability achieved for cardioembolic, followed by cryptogenic stroke.

Conclusions

We identified candidate blood-based protein biomarkers for post-stroke functional outcome involved in, e.g., NLRP3 inflammasome regulation and signaling pathways, such as TNF, JAK/STAT, MAPK, and NF-κB. These proteins warrant further study for stroke outcome prediction as well as investigations into the putative causal role for stroke outcome.

SUBMITTER: Angerfors A

PROVIDER: S-EPMC10548608 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature

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Publications

Proteomic profiling identifies novel inflammation-related plasma proteins associated with ischemic stroke outcome.

Angerfors Annelie A Brännmark Cecilia C Lagging Cecilia C Tai Kara K Månsby Svedberg Robert R Andersson Björn B Jern Christina C Stanne Tara M TM

Journal of neuroinflammation 20231004 1

<h4>Background</h4>The inflammatory response to cerebral ischemia is complex; however, most clinical studies of stroke outcome focus on a few selected proteins. We, therefore, aimed to profile a broad range of inflammation-related proteins to: identify proteins associated with ischemic stroke outcome that are independent of established clinical predictors; identify proteins subsets for outcome prediction; and perform sex and etiological subtype stratified analyses.<h4>Methods</h4>Acute-phase pla ...[more]

PMID: 37794467

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Dataset Information

Proteomic profiling identifies novel inflammation-related plasma proteins associated with ischemic stroke outcome.

Background

Methods

Results

Conclusions

Publications

Proteomic profiling identifies novel inflammation-related plasma proteins associated with ischemic stroke outcome.

Similar Datasets

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