Dataset Information

Structure of the bc₁-cbb₃ respiratory supercomplex from Pseudomonas aeruginosa.

ABSTRACT: Energy conversion by electron transport chains occurs through the sequential transfer of electrons between protein complexes and intermediate electron carriers, creating the proton motive force that enables ATP synthesis and membrane transport. These protein complexes can also form higher order assemblies known as respiratory supercomplexes (SCs). The electron transport chain of the opportunistic pathogen Pseudomonas aeruginosa is closely linked with its ability to invade host tissue, tolerate harsh conditions, and resist antibiotics but is poorly characterized. Here, we determine the structure of a P. aeruginosa SC that forms between the quinol:cytochrome c oxidoreductase (cytochrome bc₁) and one of the organism's terminal oxidases, cytochrome cbb₃, which is found only in some bacteria. Remarkably, the SC structure also includes two intermediate electron carriers: a diheme cytochrome c₄ and a single heme cytochrome c₅. Together, these proteins allow electron transfer from ubiquinol in cytochrome bc₁ to oxygen in cytochrome cbb₃. We also present evidence that different isoforms of cytochrome cbb₃ can participate in formation of this SC without changing the overall SC architecture. Incorporating these different subunit isoforms into the SC would allow the bacterium to adapt to different environmental conditions. Bioinformatic analysis focusing on structural motifs in the SC suggests that cytochrome bc₁-cbb₃ SCs also exist in other bacterial pathogens.

SUBMITTER: Di Trani JM

PROVIDER: S-EPMC10556555 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature

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Publications

Structure of the bc1-cbb3 respiratory supercomplex from Pseudomonas aeruginosa.

Di Trani Justin M JM Gheorghita Andreea A AA Turner Madison M Brzezinski Peter P Ädelroth Pia P Vahidi Siavash S Howell P Lynne PL Rubinstein John L JL

Proceedings of the National Academy of Sciences of the United States of America 20230926 40

Energy conversion by electron transport chains occurs through the sequential transfer of electrons between protein complexes and intermediate electron carriers, creating the proton motive force that enables ATP synthesis and membrane transport. These protein complexes can also form higher order assemblies known as respiratory supercomplexes (SCs). The electron transport chain of the opportunistic pathogen Pseudomonas aeruginosa is closely linked with its ability to invade host tissue, tol ...[more]

PMID: 37751552

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Project description:At present there are strong indications that Pseudomonas aeruginosa exhibits an epidemic population structure; clinical isolates are indistinguishable from environmental isolates, and they do not exhibit a specific (disease) habitat selection. However, some important issues, such as the worldwide emergence of highly transmissible P. aeruginosa clones among cystic fibrosis (CF) patients and the spread and persistence of multidrug resistant (MDR) strains in hospital wards with high antibiotic pressure, remain contentious. To further investigate the population structure of P. aeruginosa, eight parameters were analyzed and combined for 328 unrelated isolates, collected over the last 125 years from 69 localities in 30 countries on five continents, from diverse clinical (human and animal) and environmental habitats. The analysed parameters were: i) O serotype, ii) Fluorescent Amplified-Fragment Length Polymorphism (FALFP) pattern, nucleotide sequences of outer membrane protein genes, iii) oprI, iv) oprL, v) oprD, vi) pyoverdine receptor gene profile (fpvA type and fpvB prevalence), and prevalence of vii) exoenzyme genes exoS and exoU and viii) group I pilin glycosyltransferase gene tfpO. These traits were combined and analysed using biological data analysis software and visualized in the form of a minimum spanning tree (MST). We revealed a network of relationships between all analyzed parameters and non-congruence between experiments. At the same time we observed several conserved clones, characterized by an almost identical data set. These observations confirm the nonclonal epidemic population structure of P. aeruginosa, a superficially clonal structure with frequent recombinations, in which occasionally highly successful epidemic clones arise. One of these clones is the renown and widespread MDR serotype O12 clone. On the other hand, we found no evidence for a widespread CF transmissible clone. All but one of the 43 analysed CF strains belonged to a ubiquitous P. aeruginosa "core lineage" and typically exhibited the exoS(+)/exoU(-) genotype and group B oprL and oprD alleles. This is to our knowledge the first report of an MST analysis conducted on a polyphasic data set.

Dataset Information

Structure of the bc₁-cbb₃ respiratory supercomplex from Pseudomonas aeruginosa.

Publications

Structure of the <i>bc</i><sub>1</sub>-<i>cbb</i><sub>3</sub> respiratory supercomplex from <i>Pseudomonas aeruginosa</i>.

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