Ontology highlight
ABSTRACT:
SUBMITTER: McKenzie RE
PROVIDER: S-EPMC10561596 | biostudies-literature | 2022 Apr
REPOSITORIES: biostudies-literature
McKenzie Rebecca E RE Keizer Emma M EM Vink Jochem N A JNA van Lopik Jasper J Büke Ferhat F Kalkman Vera V Fleck Christian C Tans Sander J SJ Brouns Stan J J SJJ
Molecular systems biology 20220401 4
While CRISPR-Cas defence mechanisms have been studied on a population level, their temporal dynamics and variability in individual cells have remained unknown. Using a microfluidic device, time-lapse microscopy and mathematical modelling, we studied invader clearance in Escherichia coli across multiple generations. We observed that CRISPR interference is fast with a narrow distribution of clearance times. In contrast, for invaders with escaping PAM mutations we found large cell-to-cell variabili ...[more]