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Differential transcriptome response following infection of porcine ileal enteroids with species A and C rotaviruses.


ABSTRACT:

Background

Rotavirus C (RVC) is the major causative agent of acute gastroenteritis in suckling piglets, while most RVAs mostly affect weaned animals. Besides, while most RVA strains can be propagated in MA-104 and other continuous cell lines, attempts to isolate and culture RVC strains remain largely unsuccessful. The host factors associated with these unique RVC characteristics remain unknown.

Methods

In this study, we have comparatively evaluated transcriptome responses of porcine ileal enteroids infected with RVC G1P[1] and two RVA strains (G9P[13] and G5P[7]) with a focus on innate immunity and virus-host receptor interactions.

Results

The analysis of differentially expressed genes regulating antiviral immune response indicated that in contrast to RVA, RVC infection resulted in robust upregulation of expression of the genes encoding pattern recognition receptors including RIG1-like receptors and melanoma differentiation-associated gene-5. RVC infection was associated with a prominent upregulation of the most of glycosyltransferase-encoding genes except for the sialyltransferase-encoding genes which were downregulated similar to the effects observed for G9P[13].

Conclusions

Our results provide novel data highlighting the unique aspects of the RVC-associated host cellular signalling and suggest that increased upregulation of the key antiviral factors maybe one of the mechanisms responsible for RVC age-specific characteristics and its inability to replicate in most cell cultures.

SUBMITTER: Raev SA 

PROVIDER: S-EPMC10580564 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature

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Differential transcriptome response following infection of porcine ileal enteroids with species A and C rotaviruses.

Raev Sergei A SA   Raque Molly M   Kick Maryssa K MK   Saif Linda J LJ   Vlasova Anastasia N AN  

Virology journal 20231017 1


<h4>Background</h4>Rotavirus C (RVC) is the major causative agent of acute gastroenteritis in suckling piglets, while most RVAs mostly affect weaned animals. Besides, while most RVA strains can be propagated in MA-104 and other continuous cell lines, attempts to isolate and culture RVC strains remain largely unsuccessful. The host factors associated with these unique RVC characteristics remain unknown.<h4>Methods</h4>In this study, we have comparatively evaluated transcriptome responses of porci  ...[more]

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