Dataset Information

Resident Synovial Macrophages in Synovial Fluid: Implications for Immunoregulation in Infectious and Inflammatory Arthritis.

ABSTRACT:

Objectives

Resident synovial macrophages (RSM) provide immune sequestration of the joint space and are likely involved in initiation and perpetuation of the joint-specific immune response. We sought to identify RSM in synovial fluid (SF) and demonstrate migratory ability, in additional to functional changes that may perpetuate a chronic inflammatory response within joint spaces.

Methods

We recruited human patients presenting with undifferentiated arthritis in multiple clinical settings. We used flow cytometry to identify mononuclear cells in peripheral blood and SF. We used a novel transwell migration assay with human ex-vivo synovium obtained intra-operatively to validate flow cytometry findings. We used single cell RNA-sequencing (scRNA-seq) to further identify macrophage/monocyte subsets. ELISA was used to evaluate the bone-resorption potential of SF.

Results

We were able to identify a rare population of CD14^dim, OPG⁺, ZO-1⁺ cells consistent with RSM in SF via flow cytometry. These cells were relatively enriched in the SF during infectious processes, but absolutely decreased compared to healthy controls. Similar putative RSM were identified using ex vivo migration assays when MCP-1 and LPS were used as migratory stimulus. scRNA-seq revealed a population consistent with RSM transcriptionally related to CD56⁺ cytotoxic dendritic cells and IDO⁺ M2 macrophages.

Conclusion

We identified a rare cell population consistent with RSM, indicating these cells are likely migratory and able to initiate or coordinate both acute (septic) or chronic (autoimmune or inflammatory) arthritis. RSM analysis via scRNA-seq indicated these cells are M2 skewed, capable of antigen presentation, and have consistent functions in both septic and inflammatory arthritis.

SUBMITTER: Cyndari KI

PROVIDER: S-EPMC10592878 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature

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Publications

Resident Synovial Macrophages in Synovial Fluid: Implications for Immunoregulation in Infectious and Inflammatory Arthritis.

Cyndari Karen I KI Scorza Breanna M BM Zacharias Zeb R ZR Strand Leela L Mahachi Kurayi K Oviedo Juan Marcos JM Gibbs Lisa L Pessoa-Pereira Danielle D Ausdal Graham G Hendricks Dylan D Yahashiri Rika R Elkins Jacob M JM Gulbrandsen Trevor T Peterson Andrew R AR Willey Michael C MC Fairfax Keke C KC Petersen Christine A CA

bioRxiv : the preprint server for biology 20240225

<h4>Objectives</h4>Resident synovial macrophages (RSM) provide immune sequestration of the joint space and are likely involved in initiation and perpetuation of the joint-specific immune response. We sought to identify RSM in synovial fluid (SF) and demonstrate migratory ability, in additional to functional changes that may perpetuate a chronic inflammatory response within joint spaces.<h4>Methods</h4>We recruited human patients presenting with undifferentiated arthritis in multiple clinical set ...[more]

PMID: 37873090

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Dataset Information

Resident Synovial Macrophages in Synovial Fluid: Implications for Immunoregulation in Infectious and Inflammatory Arthritis.

Objectives

Methods

Results

Conclusion

Publications

Resident Synovial Macrophages in Synovial Fluid: Implications for Immunoregulation in Infectious and Inflammatory Arthritis.

Similar Datasets

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