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Direct Comparison between Forster Resonance Energy Transfer and Light-Induced Triplet-Triplet Electron Resonance Spectroscopy.

ABSTRACT: To carry out reliable and comprehensive structural investigations, the exploitation of different complementary techniques is required. Here, we report that dual triplet-spin/fluorescent labels enable the first parallel distance measurements by electron spin resonance (ESR) and Förster resonance energy transfer (FRET) on exactly the same molecules with orthogonal chromophores, allowing for direct comparison. An improved light-induced triplet-triplet electron resonance method with 2-color excitation is used, improving the signal-to-noise ratio of the data and yielding a distance distribution that provides greater insight than the single distance resulting from FRET.

SUBMITTER: Bertran A

PROVIDER: S-EPMC10603778 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature

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Direct Comparison between Förster Resonance Energy Transfer and Light-Induced Triplet-Triplet Electron Resonance Spectroscopy.

Bertran Arnau A Morbiato Laura L Sawyer Jack J Dalla Torre Chiara C Heyes Derren J DJ Hay Sam S Timmel Christiane R CR Di Valentin Marilena M De Zotti Marta M Bowen Alice M AM

Journal of the American Chemical Society 20231015 42

To carry out reliable and comprehensive structural investigations, the exploitation of different complementary techniques is required. Here, we report that dual triplet-spin/fluorescent labels enable the first parallel distance measurements by electron spin resonance (ESR) and Förster resonance energy transfer (FRET) on exactly the same molecules with orthogonal chromophores, allowing for direct comparison. An improved light-induced triplet-triplet electron resonance method with 2-color excitati ...[more]

PMID: 37839071

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