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DNA-Based Near-Infrared Voltage Sensors.

ABSTRACT: Indocyanine green (ICG) is an FDA approved dye widely used for fluorescence imaging in research, surgical navigation, and medical diagnostics. However, ICG has a few drawbacks, such as concentration-dependent aggregation and absorbance, nonspecific cellular targeting, and rapid photobleaching. Here, we report a novel DNA-based nanosensor platform that utilizes monomers of ICG and cholesterol. Using DNA origami, we can attach ICG to a DNA structure, maintaining its concentration, preserving its near-infrared (NIR) absorbance, and allowing attachment of targeting moieties. We characterized the nanosensors' absorbance, stability in blood, and voltage sensing in vitro. This study presents a novel DNA-based ICG nanosensor platform for cellular voltage sensing for future in vivo applications.

SUBMITTER: Giammanco G

PROVIDER: S-EPMC10616843 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature

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DNA-Based Near-Infrared Voltage Sensors.

Giammanco Giovanni G Veneziano Remi R Dunn Bryce B Such Nicholas N Cressman John R JR Chitnis Parag V PV

ACS sensors 20230919 10

Indocyanine green (ICG) is an FDA approved dye widely used for fluorescence imaging in research, surgical navigation, and medical diagnostics. However, ICG has a few drawbacks, such as concentration-dependent aggregation and absorbance, nonspecific cellular targeting, and rapid photobleaching. Here, we report a novel DNA-based nanosensor platform that utilizes monomers of ICG and cholesterol. Using DNA origami, we can attach ICG to a DNA structure, maintaining its concentration, preserving its n ...[more]

PMID: 37725687

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