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Transformation of Acinetobacter sp. strain BD413 by transgenic sugar beet DNA.

ABSTRACT: The ability of Acinetobacter sp. strain BD413(pFG4 delta nptII) to take up and integrate transgenic plant DNA based on homologous recombination was studied under optimized laboratory conditions. Restoration of nptII, resulting in kanamycin-resistant transformants, was observed with plasmid DNA, plant DNA, and homogenates carrying the gene nptII. Molecular analysis showed that some transformants not only restored the 317-bp deletion but also obtained additional DNA.

SUBMITTER: Gebhard F

PROVIDER: S-EPMC106187 | biostudies-literature | 1998 Apr

REPOSITORIES: biostudies-literature

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Transformation of Acinetobacter sp. strain BD413 by transgenic sugar beet DNA.

Gebhard F F Smalla K K

Applied and environmental microbiology 19980401 4

The ability of Acinetobacter sp. strain BD413(pFG4 delta nptII) to take up and integrate transgenic plant DNA based on homologous recombination was studied under optimized laboratory conditions. Restoration of nptII, resulting in kanamycin-resistant transformants, was observed with plasmid DNA, plant DNA, and homogenates carrying the gene nptII. Molecular analysis showed that some transformants not only restored the 317-bp deletion but also obtained additional DNA. ...[more]

PMID: 9546192

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