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The draft genome and pan-genome structure of Paraclostridium bifermentans strain T2 isolated from sheep faeces.

ABSTRACT: Paraclostridium bifermentans is a Gram-positive, rod-shaped bacterium that can inhabit various mesophilic environments such as soil, marine habitats, and polluted waters. Some species of Paraclostridium are reported to cause fatal infections in humans, although mechanisms and capacity for adaptation are still unknown. We hereby present the whole genome sequence data of P. bifermentans T2 strain isolated from sheep faecal matter in Potchefstroom, South Africa. DNA libraries were sequenced on the Oxford Nanopore Mk1B platform. The generated sequence data was assembled and polished using Flye assembler. Genome data analysis yielded a genome size of 2 911,782 bp, comprising of a 27.8 % G + C content. Rapid Annotation using Subsystem Technology (RAST) showed that the draft genome of this strain consists of 6 514 coding sequences (CDS). The pan-genome was defined by a total of 16 288 CDSs, grouping the strain with the genome of P. bifermentans SampleS7P1. The draft genome sequence has been deposited in NCBI GenBank with the accession number of JAUPET000000000.

SUBMITTER: Magome TG

PROVIDER: S-EPMC10622607 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature

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The draft genome and pan-genome structure of Paraclostridium bifermentans strain T2 isolated from sheep faeces.

Magome Thuto Gomolemo TG Ramatla Tsepo T Mokgokong Prudent P Thekisoe Oriel O Lekota Kgaugelo Edward KE

Data in brief 20231010

Paraclostridium bifermentans is a Gram-positive, rod-shaped bacterium that can inhabit various mesophilic environments such as soil, marine habitats, and polluted waters. Some species of Paraclostridium are reported to cause fatal infections in humans, although mechanisms and capacity for adaptation are still unknown. We hereby present the whole genome sequence data of P. bifermentans T2 strain isolated from sheep faecal matter in Potchefstroom, South Africa. DNA libraries w ...[more]

PMID: 37928322

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Project description:Microbial arsenic methylation is established as a detoxification process under aerobic conditions (converting arsenite to monomethylated arsenate) but is proposed to be a microbial warfare strategy under anoxic conditions due to the toxicity of its main product, monomethylarsonous acid (MMAs(III)). Here we leveraged a paddy soil-derived anaerobic arsenic methylator, Paraclostridium bifermentans strain EML, to gain insights into this process. Strain EML was inoculated into a series of media involving systematic dilutions of Reinforced Clostridial Broth (RCB) with 25 µM arsenite to assess the impact of growth substrate concentration on arsenic methylation. Growth curves evidenced the sensitivity of strain EML to arsenite, and arsenic speciation analysis revealed the production of MMAs(III). Concentrations of MMAs(III) and arsenic methylation gene (arsM) transcription were found to be positively correlated with RCB dilution, suggesting that substrate limitation enhances arsM gene expression and associated anaerobic arsenic methylation. We propose that growth substrate competition among microorganisms may also contribute to an increase in anaerobic arsenic methylation. This hypothesis was further evaluated in an anaerobic co-culture system involving strain EML and either wild-type Escherichia coli K-12 MG1655 (WT) or E. coli expressing the MMAs(III)-resistance gene (arsP) (ArsP E. coli). We observed increased MMAs(III) production in the presence of E. coli than its absence and growth inhibition of WT E. coli to a greater extent than ArsP E. coli, presumably due to the MMAs(III) produced by strain EML. Collectively, our findings suggest an ecological role for anaerobic arsenic methylation, highlighting the significance of microbe-microbe competition and interaction in this process.IMPORTANCEMicrobial arsenic methylation is highly active in rice paddy fields under flooded conditions, leading to increased accumulation of methylated arsenic in rice grains. In contrast to the known detoxification process for aerobic arsenic methylation, the ecological role of anaerobic arsenic methylation remains elusive and is proposed to be an antibiotic-producing process involved in microbial warfare. In this study, we interrogated a rice paddy soil-derived anaerobic arsenic-methylating bacterium, Paraclostridium bifermentans strain EML, to explore the effect of growth substrate limitation on arsenic methylation in the context of the microbial warfare hypothesis. We provide direct evidence for the role of growth substrate competition in anaerobic arsenic methylation via anaerobic prey-predator co-culture experiments. Moreover, we demonstrate a feedback loop, in which a bacterium resistant to MMAs(III) enhances its production, presumably through enhanced expression of arsM resulting from substrate limitation. Our work uncovers the complex interactions between an anaerobic arsenic methylator and its potential competitors.

Dataset Information

The draft genome and pan-genome structure of Paraclostridium bifermentans strain T2 isolated from sheep faeces.

Publications

The draft genome and pan-genome structure of <i>Paraclostridium bifermentans</i> strain T2 isolated from sheep faeces.

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