Dataset Information

Validation and development of RP-HPLC method for quantification of glibenclamide in rat plasma and its application to pharmacokinetic studies in wistar rats.

ABSTRACT: Herein, we report a novel, simple, specific, accurate and cost-friendly validated reverse phase-high performance liquid chromatographic (RP-HPLC) method for the quantification of second generation sulphonylurea based antidiabetic drug, glibenclamide (GLB) in rat plasma and its application to calculate pharmacokinetic parameters in wistar rats. The internal standard used was flufenamic acid. The chromatographic separation was conducted on C₁₈ column (250 mm × 4.6 mm x 5 μm, Agilent-Zorbax, SB) using isocratic elution with mobile phase containing Acetonitrile: Water (1:1; v/v) pH adjusted to 4.0 with 0.03 % glacial acetic acid and detected by photo-diode array as detector. Calibration curves made in the rat plasma were linear in the range of 50-1200 ng/ml with r² = 0.998. The LLOQ was 40 ng/ml. This method was effectively applied for pharmacokinetic studies of Glibenclamide following administration through oral route as solid dispersion formulation to Wistar rats. Several methods are available in the literature which can be employed for the quantification of Glibenclamide but such methods are tedious, provide lower sensitivity, less simultaneous resolution and are time-consuming. Therefore the present methods suits best for the quantification of Glibenclamide from Wistar rats.

SUBMITTER: Mir KB

PROVIDER: S-EPMC10623144 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Validation and development of RP-HPLC method for quantification of glibenclamide in rat plasma and its application to pharmacokinetic studies in wistar rats.

Mir Khalid Bashir KB Abrol Vidushi V Wani Taha U TU Jan Ishrat I Singh Nasseb N Singh Nasseb N Khan Nisar A NA Dar Alamgir A AA Sabri Sultan Rania Mohammad RM Lone Showkat A SA Iesa Mohamed A M MAM Alhag Sadeq K SK Al-Shuraym Laila A LA Helm Nawal N Al-Farga Ammar A

Heliyon 20231017 11

Herein, we report a novel, simple, specific, accurate and cost-friendly validated reverse phase-high performance liquid chromatographic (RP-HPLC) method for the quantification of second generation sulphonylurea based antidiabetic drug, glibenclamide (GLB) in rat plasma and its application to calculate pharmacokinetic parameters in wistar rats. The internal standard used was flufenamic acid. The chromatographic separation was conducted on C<sub>18</sub> column (250 mm × 4.6 mm x 5 μm, Agilent-Zor ...[more]

PMID: 37928045

Similar Datasets

Project description:Salbutamol sulfate (SAL) and prednisolone (PRD) are commonly used for treating respiratory and inflammatory conditions, yet they are frequently detected in aquatic ecosystems, posing significant risks to aquatic life and biodiversity. Despite the growing concern over pharmaceutical pollution, there is a lack of reliable and sustainable methods for quantifying these drugs in both pharmaceutical and environmental samples, as well as effective adsorbents for their removal from contaminated water. This study aims to fill this gap by developing a reliable reversed-phase high-performance liquid chromatography (RP-HPLC) method for quantifying SAL and PRD, while also creating an organoclay-activated carbon composite adsorbent for removing these drugs from water. The HPLC method was validated for linearity, precision, accuracy, robustness, and specificity, with detection limits of 1.06 μg mL-1 for SAL and 0.95 μg mL-1 for PRD. The adsorbent demonstrated high efficiency in removing both drugs, achieving maximum adsorption capacities of 731.64 mg g-1 for SAL and 888.75 mg g-1 for PRD at pH 7, with an adsorbent dose of 0.4 g and a temperature of 45 °C. Thermodynamic analysis revealed that the adsorption process is both endothermic and spontaneous. Characterization of the adsorbent using FTIR, SEM, XRD, and BET confirmed its effective structure. Adsorption followed the Langmuir model for SAL and the Sips model for PRD, with equilibrium reached within 240 minutes and the process following pseudo-second-order kinetics. Ethanol proved more effective than acetone and acetic acid for desorbing SAL, while acetone was more effective for PRD. The organoclay-activated carbon adsorbent was found to be cost-effective, offering a practical solution for large-scale water treatment. Sustainability assessments using the ComplexGAPI, BAGI, and RGB 12 algorithms highlighted its strong environmental friendliness. This research provides valuable insights for pharmaceutical quality control and the environmental remediation of pharmaceutical pollutants.

Dataset Information

Validation and development of RP-HPLC method for quantification of glibenclamide in rat plasma and its application to pharmacokinetic studies in wistar rats.

Publications

Validation and development of RP-HPLC method for quantification of glibenclamide in rat plasma and its application to pharmacokinetic studies in wistar rats.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets