Dataset Information

Local glucocorticoid synthesis regulates house dust mite-induced airway hypersensitivity in mice.

ABSTRACT:

Background

Extra-adrenal glucocorticoid (GC) synthesis at epithelial barriers, such as skin and intestine, has been shown to be important in the local regulation of inflammation. However, the role of local GC synthesis in the lung is less well studied. Based on previous studies and the uncontentious efficacy of corticosteroid therapy in asthma patients, we here investigated the role of 11β-hydroxysteroid dehydrogenase 1 (11β-HSD1/Hsd11b1)-dependent local GC reactivation in the regulation of allergic airway inflammation.

Methods

Airway inflammation in Hsd11b1-deficient and C57BL/6 wild type mice was analyzed after injection of lipopolysaccharide (LPS) and anti-CD3 antibody, and in acute and chronic models of airway hypersensitivity induced by house dust mite (HDM) extract. The role of 11β-HSD1 in normal and inflammatory conditions was assessed by high dimensional flow cytometry, histological staining, RT-qPCR analysis, ex vivo tissue cultures, GC-bioassays and protein detection by ELISA and immunoblotting.

Results

Here we show that lung tissue from Hsd11b1-deficient mice synthesized significantly less GC ex vivo compared with wild type animals in response to immune cell stimulation. We further observed a drastically aggravated phenotype in Hsd11b1-deficient mice treated with HDM extract compared to wild type animals. Besides eosinophilic infiltration, Hsd11b1-deficient mice exhibited aggravated neutrophilic infiltration caused by a strong Th17-type immune response.

Conclusion

We propose an important role of 11β-HSD1 and local GC in regulating Th17-type rather than Th2-type immune responses in HDM-induced airway hypersensitivity in mice by potentially controlling Toll-like receptor 4 (TLR4) signaling and cytokine/chemokine secretion by airway epithelial cells.

SUBMITTER: Merk VM

PROVIDER: S-EPMC10626452 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature

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Publications

Local glucocorticoid synthesis regulates house dust mite-induced airway hypersensitivity in mice.

Merk Verena M VM Phan Truong San TS Wiedmann Alice A Hardy Rowan S RS Lavery Gareth G GG Brunner Thomas T

Frontiers in immunology 20231023

<h4>Background</h4>Extra-adrenal glucocorticoid (GC) synthesis at epithelial barriers, such as skin and intestine, has been shown to be important in the local regulation of inflammation. However, the role of local GC synthesis in the lung is less well studied. Based on previous studies and the uncontentious efficacy of corticosteroid therapy in asthma patients, we here investigated the role of 11β-hydroxysteroid dehydrogenase 1 (11β-HSD1/<i>Hsd11b1</i>)-dependent local GC reactivation in the reg ...[more]

PMID: 37936704

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Dataset Information

Local glucocorticoid synthesis regulates house dust mite-induced airway hypersensitivity in mice.

Background

Methods

Results

Conclusion

Publications

Local glucocorticoid synthesis regulates house dust mite-induced airway hypersensitivity in mice.

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