C-terminal modification and functionalization of proteins via a self-cleavage tag triggered by a small molecule.

ABSTRACT: The precise modification or functionalization of the protein C-terminus is essential but full of challenges. Herein, a chemical approach to modify the C-terminus is developed by fusing a cysteine protease domain on the C-terminus of the protein of interest, which could achieve the non-enzymatic C-terminal functionalization by InsP₆-triggered cysteine protease domain self-cleavage. This method demonstrates a highly efficient way to achieve protein C-terminal functionalization and is compatible with a wide range of amine-containing molecules and proteins. Additionally, a reversible C-terminal de-functionalization is found by incubating the C-terminal modified proteins with cysteine protease domain and InsP₆, providing a tool for protein functionalization and de-function

SUBMITTER: Zeng Y 

PROVIDER: S-EPMC10630284 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature