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Protocol for simultaneous analysis of peripheral and intratumoral lymphocyte function by flow cytometry.


ABSTRACT: Here, we present a protocol for the simultaneous analysis of peripheral and intratumoral lymphocyte function by flow cytometry. Using tumor and blood samples from patients with colorectal cancer, we describe steps for tumor digestion, pre-labeling of the tumor-infiltrating leukocytes (TILs), and activation and labeling of the total cells (TILs and whole blood cells). For complete details on the use and execution of this protocol, please refer to Thibaudin et al.1.

SUBMITTER: Daumoine S 

PROVIDER: S-EPMC10630783 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature

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Protocol for simultaneous analysis of peripheral and intratumoral lymphocyte function by flow cytometry.

Daumoine Susy S   Hampe Léa L   Limagne Emeric E   Ghiringhelli François F   Thibaudin Marion M  

STAR protocols 20231031 4


Here, we present a protocol for the simultaneous analysis of peripheral and intratumoral lymphocyte function by flow cytometry. Using tumor and blood samples from patients with colorectal cancer, we describe steps for tumor digestion, pre-labeling of the tumor-infiltrating leukocytes (TILs), and activation and labeling of the total cells (TILs and whole blood cells). For complete details on the use and execution of this protocol, please refer to Thibaudin et al.<sup>1</sup>. ...[more]

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