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Culture conditions control expression of the genes for aflatoxin and sterigmatocystin biosynthesis in Aspergillus parasiticus and A. nidulans.

ABSTRACT: High temperature and nitrate supported gene expression for sterigmatocystin biosynthesis in Aspergillus nidulans; ammonium did not. Homologous genes for aflatoxin biosynthesis in A. parasiticus showed the opposite transcript expression pattern, suggesting that the two mycotoxins are regulated differently. The aflR gene is postulated to require additional genetic elements to effect its own activation by the different culture conditions. A patulin polyketide synthase (PKS) gene was found to be regulated differently than the aflatoxin PKS. Thus, the biosyntheses of structurally similar compounds in these two fungi appear to be regulated very differently.

SUBMITTER: Feng GH

PROVIDER: S-EPMC106313 | biostudies-literature | 1998 Jun

REPOSITORIES: biostudies-literature

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Culture conditions control expression of the genes for aflatoxin and sterigmatocystin biosynthesis in Aspergillus parasiticus and A. nidulans.

Feng G H GH Leonard T J TJ

Applied and environmental microbiology 19980601 6

High temperature and nitrate supported gene expression for sterigmatocystin biosynthesis in Aspergillus nidulans; ammonium did not. Homologous genes for aflatoxin biosynthesis in A. parasiticus showed the opposite transcript expression pattern, suggesting that the two mycotoxins are regulated differently. The aflR gene is postulated to require additional genetic elements to effect its own activation by the different culture conditions. A patulin polyketide synthase (PKS) gene was found to be reg ...[more]

PMID: 9603849

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Project description:O-Methyltransferase I catalyzes both the conversion of demethylsterigmatocystin to sterigmatocystin and the conversion of dihydrodemethylsterigmatocystin to dihydrosterigmatocystin during aflatoxin biosynthesis. In this study, both genomic cloning and cDNA cloning of the gene encoding O-methyltransferase I were accomplished by using PCR strategies, such as conventional PCR based on the N-terminal amino acid sequence of the purified enzyme, 5' and 3' rapid amplification of cDNA ends PCR, and thermal asymmetric interlaced PCR (TAIL-PCR), and genes were sequenced by using Aspergillus parasiticus NIAH-26. A comparison of the genomic sequences with the cDNA of the dmtA region revealed that the coding region is interrupted by three short introns. The cDNA of the dmtA gene is 1,373 bp long and encodes a 386-amino-acid protein with a deduced molecular weight of 43,023, which is consistent with the molecular weight of the protein determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The C-terminal half of the deduced protein exhibits 76.3% identity with the coding region of the Aspergillus nidulans StcP protein, whereas the N-terminal half of dmtA exhibits 73.0% identity with the 5' flanking region of the stcP gene, suggesting that translation of the stcP gene may start at a site upstream from methionine that is different from the site that has been suggested previously. Also, an examination of the 5' and 3' flanking regions of the dmtA gene in which TAIL-PCR was used demonstrated that the dmtA gene is located in the aflatoxin biosynthesis cluster between (and in the same orientation as) the omtA and ord-2 genes. Northern blotting revealed that expression of the dmtA gene is influenced by both medium composition and culture temperature and that the pattern correlates with the patterns observed for other genes in the aflatoxin gene cluster. Furthermore, Southern blotting and PCR analyses of the dmtA gene showed that a dmtA homolog is present in Aspergillus oryzae SYS-2.

Dataset Information

Culture conditions control expression of the genes for aflatoxin and sterigmatocystin biosynthesis in Aspergillus parasiticus and A. nidulans.

Publications

Culture conditions control expression of the genes for aflatoxin and sterigmatocystin biosynthesis in Aspergillus parasiticus and A. nidulans.

Similar Datasets

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