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A sensitive LC-MS assay using derivatization with boron trifluoride to quantify curcuminoids in biological samples.

ABSTRACT: A procedure is described to measure curcumin (C), demethoxycurcumin (DMC), bisdemethoxycurcumin (BDMC), tetrahydrocurcumim (TC) and their glucuronidated metabolites (CG, DMCG, and BDMCG) in plasma, brain, liver and tumor samples. The procedure involves converting the analytes to their boron difluoride derivatives and analyzing them by combined liquid chromatography coupled to an ion trap mass spectrometer operating in the negative ion MSn scan mode. The method has superb limits of detection of 0.01 nM for all curcuminoids and 0.5 nM for TC and the glucuroniated metabolites, and several representative chromatograms of biological samples containing these analytes are provided. In addition, the pharmacokinetic profile of these compounds in one human who daily consumed an over-the-counter curcuminoid product shows the peak and changes in circulating concentrations achieved by this mode of administration.

SUBMITTER: Yoon AJ 

PROVIDER: S-EPMC10631463 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature

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A sensitive LC-MS assay using derivatization with boron trifluoride to quantify curcuminoids in biological samples.

Yoon Alexander J AJ   Wu Haiqiang H   Pan Roy D RD   Teter Bruce B   Cipolla Jack J   Chang Edwin E   Avila Luis Z LZ   Basak Saroj K SK   Srivatsan Eri S ES   Wang Marilene B MB   Cole Greg M GM   Frautschy Sally A SA   Hampton Phillip D PD   Faull Kym F KF  

Analytical biochemistry 20200217

A procedure is described to measure curcumin (C), demethoxycurcumin (DMC), bisdemethoxycurcumin (BDMC), tetrahydrocurcumim (TC) and their glucuronidated metabolites (CG, DMCG, and BDMCG) in plasma, brain, liver and tumor samples. The procedure involves converting the analytes to their boron difluoride derivatives and analyzing them by combined liquid chromatography coupled to an ion trap mass spectrometer operating in the negative ion MS<sup>n</sup> scan mode. The method has superb limits of det  ...[more]

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