Ontology highlight
ABSTRACT:
SUBMITTER: McGee AV
PROVIDER: S-EPMC10634825 | biostudies-literature | 2023 Oct
REPOSITORIES: biostudies-literature
McGee Abby V AV Liu Yanjing V YV Griffith Audrey L AL Szegletes Zsofia M ZM Wen Bronte B Kraus Carolyn C Miller Nathan W NW Steger Ryan J RJ Velasco Berta Escude BE Bosch Justin A JA Zirin Jonathan D JD Viswanatha Raghuvir R Sontheimer Erik J EJ Goodale Amy A Greene Matthew A MA Green Thomas M TM Doench John G JG
bioRxiv : the preprint server for biology 20231027
The diversity of CRISPR systems, coupled with scientific ingenuity, has led to an explosion of applications; however, to test newly-described innovations in their model systems, researchers typically embark on cumbersome, one-off cloning projects to generate custom reagents that are optimized for their biological questions. Here, we leverage Golden Gate cloning to create the Fragmid toolkit, a modular set of CRISPR cassettes and delivery technologies, along with a web portal, resulting in a comb ...[more]