Heterologous expression, purification, and characterization of a highly active xylose reductase from Neurospora crassa.
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ABSTRACT:
SUBMITTER: Woodyer R
PROVIDER: S-EPMC1065158 | biostudies-literature | 2005 Mar
REPOSITORIES: biostudies-literature
Publications
Heterologous expression, purification, and characterization of a highly active xylose reductase from Neurospora crassa.
Woodyer Ryan R Simurdiak Michael M van der Donk Wilfred A WA Zhao Huimin H
Applied and environmental microbiology 20050301 3
A xylose reductase (XR) gene was identified from the Neurospora crassa whole-genome sequence, expressed heterologously in Escherichia coli, and purified as a His6-tagged fusion in high yield. This enzyme is one of the most active XRs thus far characterized and may be used for the in vitro production of xylitol. ...[more]
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