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AePUb promoter length modulates gene expression in Aedes aegypti.


ABSTRACT: Molecular tools for modulating transgene expression in Aedes aegypti are few. Here we demonstrate that adjustments to the AePUb promoter length can alter expression levels of two reporter proteins in Ae. aegypti cell culture and in mosquitoes. This provides a simple means for increasing or decreasing expression of a gene of interest and easy translation from cells to whole insects.

SUBMITTER: Anderson MAE 

PROVIDER: S-EPMC10663553 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature

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AePUb promoter length modulates gene expression in Aedes aegypti.

Anderson Michelle A E MAE   Leftwich Philip T PT   Wilson Ray R   Paladino Leonela Z Carabajal LZC   Basu Sanjay S   Rooney Sara S   Adelman Zach N ZN   Alphey Luke L  

Scientific reports 20231121 1


Molecular tools for modulating transgene expression in Aedes aegypti are few. Here we demonstrate that adjustments to the AePUb promoter length can alter expression levels of two reporter proteins in Ae. aegypti cell culture and in mosquitoes. This provides a simple means for increasing or decreasing expression of a gene of interest and easy translation from cells to whole insects. ...[more]

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