Ontology highlight
ABSTRACT:
SUBMITTER: Jeung JH
PROVIDER: S-EPMC10669037 | biostudies-literature | 2023 Oct
REPOSITORIES: biostudies-literature
Jeung Jae Hoon JH Han Hyogu H Lee Chang Yeol CY Ahn Jun Ki JK
Biosensors 20231030 11
This study presents a technique for detecting 3'-5' exonuclease activity through the use of CRISPR/Cas12a. These enzymes, including 3'-5' exonuclease (Exo III), perform crucial roles in various cellular processes and are associated with life expectancy. However, imbalances in their expression can increase susceptibility to diseases such as cancer, particularly under prolonged stress. In this study, an activator sequence of CRISPR/Cas12a was constructed on the 5'-end of a hairpin probe (HP), form ...[more]