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Heterologous expression of bacterial dehydrin gene in Arabidopsis thaliana promotes abiotic stress tolerance.

ABSTRACT: Salinity, low temperature, and drought are major environmental factors in agriculture leading to reduced crop yield. Dehydrins (DHNs) are induced transcriptionally during cellular dehydration and accumulate in different tissues during abiotic stresses. Here we isolated and characterized a bacterial gene BG757 in Arabidopsis, encoding a putative dehydrin type protein. ABA induces the expression of various dehydrins in plants, therefore, to elucidate the potential role, ABA sensitivity was examined in Arabidopsis transgenic lines expressing BG757. Interestingly, BG757-expressing plants showed hypersensitivity towards NaCl and ABA during seed germination. In addition to germination, BG757-expressing plants also showed root growth retardation in the presence of ABA and NaCl when compared with wild type (WT), suggesting that BG757 positively regulate salt stress and ABA response. Furthermore, BG757-expressing plants showed significant drought tolerance compared with WT. Consistent with drought tolerance, expression levels of stress inducible genes (DREB2A, RD22, RD26, LEA7 and SOS1) were strongly upregulated in transgenic plants compared with WT. All together these results suggest that heterologous expression of bacterial gene, BG757 in plants promotes resistance to environmental stresses.

Supplementary information

The online version contains supplementary material available at 10.1007/s12298-023-01358-w.

SUBMITTER: Khan NZ

PROVIDER: S-EPMC10678877 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature

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Heterologous expression of bacterial dehydrin gene in <i>Arabidopsis thaliana</i> promotes abiotic stress tolerance.

Khan Nadir Zaman NZ Ali Akhtar A Ali Waqar W Aasim Muhammad M Khan Tariq T Khan Zaryab Z Munir Iqbal I

Physiology and molecular biology of plants : an international journal of functional plant biology 20230920 9

Salinity, low temperature, and drought are major environmental factors in agriculture leading to reduced crop yield. Dehydrins (DHNs) are induced transcriptionally during cellular dehydration and accumulate in different tissues during abiotic stresses. Here we isolated and characterized a bacterial gene <i>BG757</i> in Arabidopsis, encoding a putative dehydrin type protein. ABA induces the expression of various dehydrins in plants, therefore, to elucidate the potential role, ABA sensitivity was ...[more]

PMID: 38024953

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