Unknown

Dataset Information

0

Degradation of 1,3-dichloropropene by pseudomonas cichorii 170.


ABSTRACT: The gram-negative bacterium Pseudomonas cichorii 170, isolated from soil that was repeatedly treated with the nematocide 1, 3-dichloropropene, could utilize low concentrations of 1, 3-dichloropropene as a sole carbon and energy source. Strain 170 was also able to grow on 3-chloroallyl alcohol, 3-chloroacrylic acid, and several 1-halo-n-alkanes. This organism produced at least three different dehalogenases: a hydrolytic haloalkane dehalogenase specific for haloalkanes and two 3-chloroacrylic acid dehalogenases, one specific for cis-3-chloroacrylic acid and the other specific for trans-3-chloroacrylic acid. The haloalkane dehalogenase and the trans-3-chloroacrylic acid dehalogenase were expressed constitutively, whereas the cis-3-chloroacrylic acid dehalogenase was inducible. The presence of these enzymes indicates that 1, 3-dichloropropene is hydrolyzed to 3-chloroallyl alcohol, which is oxidized in two steps to 3-chloroacrylic acid. The latter compound is then dehalogenated, probably forming malonic acid semialdehyde. The haloalkane dehalogenase gene, which is involved in the conversion of 1,3-dichloropropene to 3-chloroallyl alcohol, was cloned and sequenced, and this gene turned out to be identical to the previously studied dhaA gene of the gram-positive bacterium Rhodococcus rhodochrous NCIMB13064. Mutants resistant to the suicide substrate 1,2-dibromoethane lacked haloalkane dehalogenase activity and therefore could not utilize haloalkanes for growth. PCR analysis showed that these mutants had lost at least part of the dhaA gene.

SUBMITTER: Poelarends GJ 

PROVIDER: S-EPMC106795 | biostudies-literature | 1998 Aug

REPOSITORIES: biostudies-literature

altmetric image

Publications

Degradation of 1,3-dichloropropene by pseudomonas cichorii 170.

Poelarends G J GJ   Wilkens M M   Larkin M J MJ   van Elsas J D JD   Janssen D B DB  

Applied and environmental microbiology 19980801 8


The gram-negative bacterium Pseudomonas cichorii 170, isolated from soil that was repeatedly treated with the nematocide 1, 3-dichloropropene, could utilize low concentrations of 1, 3-dichloropropene as a sole carbon and energy source. Strain 170 was also able to grow on 3-chloroallyl alcohol, 3-chloroacrylic acid, and several 1-halo-n-alkanes. This organism produced at least three different dehalogenases: a hydrolytic haloalkane dehalogenase specific for haloalkanes and two 3-chloroacrylic acid  ...[more]

Similar Datasets

| PRJNA319731 | ENA
| PRJDB9457 | ENA
2017-02-01 | GSE86142 | GEO
| PRJNA232591 | ENA
| PRJNA338910 | ENA
| S-EPMC4968640 | biostudies-literature
| S-EPMC2760366 | biostudies-literature
| S-EPMC8632607 | biostudies-literature
| S-EPMC2330126 | biostudies-literature
| S-EPMC2885751 | biostudies-literature