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Bispecific Antibody Detection Using Antigen-Conjugated Synthetic Nucleic Acid Strands.

ABSTRACT: We report here the development of two different sensing strategies based on the use of antigen-conjugated nucleic acid strands for the detection of a bispecific antibody against the tumor-related proteins Mucin1 and epidermal growth factor receptor. Both approaches work well in serum samples (nanomolar sensitivity), show high specificity against the two monospecific antibodies, and are rapid. The results presented here demonstrate the versatility of DNA-based platforms for the detection of bispecific antibodies and could represent a versatile alternative to other more reagent-intensive and time-consuming analytical approaches.

SUBMITTER: Mariottini D

PROVIDER: S-EPMC10683503 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature

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Bispecific Antibody Detection Using Antigen-Conjugated Synthetic Nucleic Acid Strands.

Mariottini Davide D Bracaglia Sara S Barbero Luca L Fuchs Sebastian W SW Saal Christoph C Moniot Sebastien S Knuehl Christine C Baranda Lorena L Ranallo Simona S Ricci Francesco F

ACS sensors 20231019 11

We report here the development of two different sensing strategies based on the use of antigen-conjugated nucleic acid strands for the detection of a bispecific antibody against the tumor-related proteins Mucin1 and epidermal growth factor receptor. Both approaches work well in serum samples (nanomolar sensitivity), show high specificity against the two monospecific antibodies, and are rapid. The results presented here demonstrate the versatility of DNA-based platforms for the detection of bispe ...[more]

PMID: 37856082

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