Dataset Information

Cuproptosis-related gene DLAT serves as a prognostic biomarker for immunotherapy in clear cell renal cell carcinoma: multi-database and experimental verification.

ABSTRACT:

Objective

Renal clear cell carcinoma (ccRCC) is the most common type of renal cancer. Here we aim to explore the prognosis and immunotherapeutic value of copper death-related gene Dihydrolipoamide S-acetyltransferase (DLAT) in ccRCC.

Methods

The mRNA and protein expressions and methylation level of DLAT, as well as the relation of DLAT to survival prognosis, clinical characteristics, biological function, and immune microenvironment and responses in patients with ccRCC were evaluated using multiple databases. In addition, 75 paired ccRCC tissue samples and 3 kinds of cell lines were tested for experimental validation.

Results

Bioinformatics analysis of multiple databases, qRT-PCR, and western blot verified that DLAT expression in ccRCC was lower than that in paracancerous tissues. Patients with low expression of DLAT had a lower survival rate, worse clinical prognosis, stronger immune cell infiltration and expression of immunosuppressive points, and higher tumor immune dysfunction and exclusion (TIDE) scores.

Conclusions

DLAT was identified as an independent prognostic factor in ccRCC and was closely related to the prognosis and immune responses of patients with ccRCC.

SUBMITTER: Huang S

PROVIDER: S-EPMC10683628 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature

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Cuproptosis-related gene <i>DLAT</i> serves as a prognostic biomarker for immunotherapy in clear cell renal cell carcinoma: multi-database and experimental verification.

Huang Shuaishuai S Cai Congbo C Zhou Kena K Wang Xue X Wang Xue X Cen Dong D Weng Guobin G

Aging 20231107 21

<h4>Objective</h4>Renal clear cell carcinoma (ccRCC) is the most common type of renal cancer. Here we aim to explore the prognosis and immunotherapeutic value of copper death-related gene <i>Dihydrolipoamide S-acetyltransferase</i> (<i>DLAT)</i> in ccRCC.<h4>Methods</h4>The mRNA and protein expressions and methylation level of <i>DLAT</i>, as well as the relation of <i>DLAT</i> to survival prognosis, clinical characteristics, biological function, and immune microenvironment and responses in pati ...[more]

PMID: 37938155

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