PEP-Patch: Electrostatics in Protein-Protein Recognition, Specificity, and Antibody Developability.

ABSTRACT: The electrostatic properties of proteins arise from the number and distribution of polar and charged residues. Electrostatic interactions in proteins play a critical role in numerous processes such as molecular recognition, protein solubility, viscosity, and antibody developability. Thus, characterizing and quantifying electrostatic properties of a protein are prerequisites for understanding these processes. Here, we present PEP-Patch, a tool to visualize and quantify the electrostatic potential on the protein surface in terms of surface patches, denoting separated areas of the surface with a common physical property. We highlight its applicability to elucidate protease substrate specificity and antibody-antigen recognition and predict heparin column retention times of antibodies as an indicator of pharmacokinetics.

SUBMITTER: Hoerschinger VJ 

PROVIDER: S-EPMC10685443 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature