Dataset Information

Differential Gene Expressions of CALM1, PSMD6, and AK124742 Long Noncoding RNA in Cumulus Cells from Polycystic Ovary Syndrome Patients versus Normal Control Women.

ABSTRACT:

Background

One of the well-known causes of subfertility is polycystic ovary syndrome (PCOS). Genetic components play a critical role in the etiology of PCOS. The recognition of differentially expressed genes in PCOS patients might provide a better understanding of the pathophysiology of this syndrome and paves the way for novel therapeutics. Gene expression profiles in cumulus cells (CCs) could be used as biological criteria for embryo competence and their analysis might lead to important molecular information about embryo quality. CALM1, PSMD6, and AK124742 are three well-known genes associated with embryo development. Therefore, the objective of this study was to compare the expression of CALM1, PSMD6, and AK124742 genes in the CCs of infertile PCOS patients with their expression in the CCs of the donor fertile group.

Materials and methods

CCs were collected from the follicular fluid of 33 patients with PCOS as the experimental group and 33 cumulus donor women who were referred to the infertility center for egg donation as the control group. CCs were frozen until genetic testing. The expression of CALM1, PSMD6, and AK124742 genes was detected by real-time polymerase chain reaction.

Results

CALM1 and AK124742 gene expressions significantly increased (CALM1 P = 0.003) (AK124742 P = 0.000) and PSMD6 expression significantly decreased (P = 0.002) in the PCOS group compared to the cumulus donor (control) group.

Conclusion

Therefore, our research findings suggest that the potential impact of Polycystic Ovary Syndrome (PCOS) on fertility could be attributed to modifications in the expression levels of genes that affect the reproductive.

SUBMITTER: Akbari A

PROVIDER: S-EPMC10699245 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature

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Publications

Differential Gene Expressions of <i>CALM1</i>, <i>PSMD6</i>, and <i>AK124742</i> Long Noncoding RNA in Cumulus Cells from Polycystic Ovary Syndrome Patients versus Normal Control Women.

Akbari Ali A Aboutorabi Roshanak R Kazemi Mohammad M Borzouie Zahra Z Feizi Awat A Naghshineh Elham E Mostafavi Fatemesadat F

Advanced biomedical research 20231028

<h4>Background</h4>One of the well-known causes of subfertility is polycystic ovary syndrome (PCOS). Genetic components play a critical role in the etiology of PCOS. The recognition of differentially expressed genes in PCOS patients might provide a better understanding of the pathophysiology of this syndrome and paves the way for novel therapeutics. Gene expression profiles in cumulus cells (CCs) could be used as biological criteria for embryo competence and their analysis might lead to importan ...[more]

PMID: 38073758

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