Unknown

Dataset Information

0

Histone N-tails modulate sequence-specific positioning of nucleosomes.


ABSTRACT: The precise mechanisms governing sequence-dependent positioning of nucleosomes on DNA remain unknown in detail. Existing algorithms, taking into account the sequence-dependent deformability of DNA and its interactions with the histone globular domains, predict rotational setting of only 65% of human nucleosomes mapped in vivo. To uncover novel factors responsible for the nucleosome positioning, we analyzed potential involvement of the histone N-tails in this process. To this aim, we reconstituted the H2A/H4 N-tailless nucleosomes on human BRCA1 DNA (~100 kb) and compared their positions and sequences with those of the wild-type nucleosomes. In the case of H2A tailless nucleosomes, the AT content of DNA sequences is changed locally at superhelical location (SHL) ±4, while maintaining the same rotational setting as their wild-type counterparts. Conversely, the H4 tailless nucleosomes display widespread changes of the AT content near SHL ±1 and SHL ±2, where the H4 N-tails interact with DNA. Furthermore, a substantial number of H4 tailless nucleosomes exhibit rotational setting opposite to that of the wild-type nucleosomes. Thus, our findings strongly suggest that the histone N-tails are operative in selection of nucleosome positions, which may have wide-ranging implications for epigenetic modulation of chromatin states.

SUBMITTER: Nikitina T 

PROVIDER: S-EPMC10705531 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature

altmetric image

Publications

Histone N-tails modulate sequence-specific positioning of nucleosomes.

Nikitina Tatiana T   Guiblet Wilfried M WM   Cui Feng F   Zhurkin Victor B VB  

bioRxiv : the preprint server for biology 20231130


The precise mechanisms governing sequence-dependent positioning of nucleosomes on DNA remain unknown in detail. Existing algorithms, taking into account the sequence-dependent deformability of DNA and its interactions with the histone globular domains, predict rotational setting of only 65% of human nucleosomes mapped <i>in vivo</i>. To uncover novel factors responsible for the nucleosome positioning, we analyzed potential involvement of the histone N-tails in this process. To this aim, we recon  ...[more]

Similar Datasets

2024-01-03 | GSE252427 | GEO
| PRJNA1060631 | ENA
| S-EPMC8174689 | biostudies-literature
| S-EPMC8421395 | biostudies-literature
| S-EPMC3821030 | biostudies-literature
| S-EPMC5295774 | biostudies-literature
| S-EPMC9376062 | biostudies-literature
| S-EPMC6120228 | biostudies-literature
| S-EPMC2000439 | biostudies-literature
| S-EPMC5198986 | biostudies-literature