Variation of the ribosomal operon 16S-23S gene spacer region in representatives of Salmonella enterica subspecies.

ABSTRACT: The 16S-23S spacer regions of two ribosomal operons (rrnA and rrnE) have been sequenced in seven representatives of the Salmonella enterica subspecies. Isolated nucleotide substitutions were found at the same sites as in Escherichia coli but the number of polymorphic sites was much larger, as could be expected for a more heterogeneous species. Still, as in E. coli, most of the variation found was due to insertions and/or deletions affecting blocks of nucleotides generally located at equivalent regions of the putative secondary structure for both species. Isolated polymorphic sites generated phylogenetic trees generally consistent with the subspecies structure and the accepted relationships among the subspecies. However, the sequences of rrnE put subspecies I closer to E. coli K-12 than to

SUBMITTER: Perez Luz S 

PROVIDER: S-EPMC107142 | biostudies-literature | 1998 Apr

REPOSITORIES: biostudies-literature