Ontology highlight
ABSTRACT:
SUBMITTER: Meng X
PROVIDER: S-EPMC10718767 | biostudies-literature | 2022
REPOSITORIES: biostudies-literature
Meng Xiaoyi X Li Min M Qiao Ming M Li Xue X Yang Jianhua J Hu Junping J
Evidence-based complementary and alternative medicine : eCAM 20221219
This study explored the effects of Hugan Buzure (HBR) on cell apoptosis and autophagy in hepatocellular carcinoma (HCC) and the molecular mechanisms of the PI3K/Akt/mTOR signaling pathway. HepG2 and Huh7 cell viability was detected by the tetramethylazolium salt colorimetric (MTT) method. Cell proliferation was measured using the colony formation method. Hoechst 33258 staining and flow cytometry were employed to detect apoptosis. In addition, immunofluorescence was carried out to evaluate the ex ...[more]