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Deep-tissue SWIR imaging using rationally designed small red-shifted near-infrared fluorescent protein.

ABSTRACT: Applying rational design, we developed 17 kDa cyanobacteriochrome-based near-infrared (NIR-I) fluorescent protein, miRFP718nano. miRFP718nano efficiently binds endogenous biliverdin chromophore and brightly fluoresces in mammalian cells and tissues. miRFP718nano has maximal emission at 718 nm and an emission tail in the short-wave infrared (SWIR) region, allowing deep-penetrating off-peak fluorescence imaging in vivo. The miRFP718nano structure reveals the molecular basis of its red shift. We demonstrate superiority of miRFP718nano-enabled SWIR imaging over NIR-I imaging of microbes in the mouse digestive tract, mammalian cells injected into the mouse mammary gland and NF-kB activity in a mouse model of liver inflammation.

SUBMITTER: Oliinyk OS

PROVIDER: S-EPMC10725253 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature

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Deep-tissue SWIR imaging using rationally designed small red-shifted near-infrared fluorescent protein.

Oliinyk Olena S OS Ma Chenshuo C Pletnev Sergei S Baloban Mikhail M Taboada Carlos C Sheng Huaxin H Yao Junjie J Verkhusha Vladislav V VV

Nature methods 20221201 1

Applying rational design, we developed 17 kDa cyanobacteriochrome-based near-infrared (NIR-I) fluorescent protein, miRFP718nano. miRFP718nano efficiently binds endogenous biliverdin chromophore and brightly fluoresces in mammalian cells and tissues. miRFP718nano has maximal emission at 718 nm and an emission tail in the short-wave infrared (SWIR) region, allowing deep-penetrating off-peak fluorescence imaging in vivo. The miRFP718nano structure reveals the molecular basis of its red shift. We de ...[more]

PMID: 36456785

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