Dataset Information

Selenium biofortification improves bioactive composition and antioxidant status in Plantago ovata Forsk., a medicinal plant.

ABSTRACT:

Background

Selenium (Se) is an essential micronutrient for humans, but its deficiency as well as toxicity affects large number of people worldwide. Plantago ovata, a commercially important medicinal plant, is mainly cultivated in western regions of India, where elevated levels of Se have been found in soil. Thus, we evaluated the potential of Se biofortification in P. ovata via phytoremediation and its effect on the bioactive composition.

Results

The results showed a significant alteration in various morphological and physiological parameters in a dose-dependent manner. The 10 µM Se dose improved seedling height, biomass and total chlorophyll content. There was a gradual increase in total Se content, with highest accumulation of 457.65 µg/g FW at 500 µM Se treatment. Se positively affected the antioxidative metabolism which was measured from the change in total antioxidant capacity, radical scavenging activity and Metallothionein 2 expression. Increasing levels of Se also affected the PAL activity, total polyphenol and flavonoid content. Caffeic acid, Coumaric acid and Rutin were found to be the most abundant phenolic compounds.

Conclusions

Low levels of selenium (below 50 µM) can successfully improve Se accumulation and elicit production of various polyphenols without hampering plant growth. Thus, Se fortification of P. ovata seedlings via phytoremediation appears to be a feasible and efficient way to enhance its nutraceutical value in dietary products.

SUBMITTER: Dey S

PROVIDER: S-EPMC10729483 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature

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Publications

Selenium biofortification improves bioactive composition and antioxidant status in Plantago ovata Forsk., a medicinal plant.

Dey Sankalan S Raychaudhuri Sarmistha Sen SS

Genes and environment : the official journal of the Japanese Environmental Mutagen Society 20231219 1

<h4>Background</h4>Selenium (Se) is an essential micronutrient for humans, but its deficiency as well as toxicity affects large number of people worldwide. Plantago ovata, a commercially important medicinal plant, is mainly cultivated in western regions of India, where elevated levels of Se have been found in soil. Thus, we evaluated the potential of Se biofortification in P. ovata via phytoremediation and its effect on the bioactive composition.<h4>Results</h4>The results showed a significant a ...[more]

PMID: 38111072

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Project description:BackgroundPlants experience severe physiological stress from heavy metal pollution caused by improper discarding of the industrial wastes. Hexavalent chromium [Cr (VI)] is one of the major heavy metal pollutants in India and is present particularly in some regions where Plantago ovata grows to a great extent. This study was aimed at finding the effects of Cr (VI) on P. ovata and manoeuvres of the plant to combat such heavy metal exposure in vitro.MethodsPotassium dichromate was used as a source of Cr (VI) to induce the heavy metal stress. Range of Cr (VI) sublethal doses [0 mM (control), 0.1 mM, 0.3 mM, 0.5 mM, 1 mM, 1.5 mM and1.8 mM] was used to observe its effect on the plant. The seeds of the plant were grown on sucrose-agar media with different concentrations of potassium dichromate, and ten-day old seedlings were then harvested and examined.ResultsThe germination rate reduced below 50% at 1.9 mM Cr (VI) concentration and thus, 0 mM-1.8 mM concentration ranges were found to be suitable for sublethal dose. Morphological changes namely, reduction of the shoot-root length and multiple root development were caused by Cr (VI) in a dose-dependent manner. The plant showed elevated responses against Cr (VI), up to 1.5 mM (10 days treated) in terms of increasing accumulation of secondary metabolites like polyphenols, chlorophyll content (chlorophyll a, b and total chlorophyll), carotenoids and total antioxidant activity. DPPH radical scavenging activity along with malondialdehyde (MDA) content was not significantly elevated with the increase in Cr (VI) concentration indicating that the lipid peroxidation rate within the tissue was low. Phenylalanine ammonia lyase (PAL) and polyphenol oxidase (PPO) gene expressions were upregulated by 1 mM Cr (VI) concentration, which decreased at higher concentrations. The atomic absorption spectroscopy analysis also showed significant accumulation of Cr (VI) in the shoot and root with an increase in the potassium dichromate concentration.ConclusionCr (VI) reduced the shoot-root length and seed germination in a dose-dependent manner. The plant system tried to combat the Cr (VI) stress by upregulating the stress response genes in the phenylpropanoid pathway along with an increase in polyphenol and antioxidant contents, which were evident from the lowering of lipid peroxidation rate and increase in PAL and PPO gene expressions.

Dataset Information

Selenium biofortification improves bioactive composition and antioxidant status in Plantago ovata Forsk., a medicinal plant.

Background

Results

Conclusions

Publications

Selenium biofortification improves bioactive composition and antioxidant status in Plantago ovata Forsk., a medicinal plant.

Similar Datasets

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