Ontology highlight
ABSTRACT:
SUBMITTER: Feng S
PROVIDER: S-EPMC1074391 | biostudies-literature | 2005 Apr
REPOSITORIES: biostudies-literature
Feng Sunlian S Ku Karin K Hodzic Emir E Lorenzana Edward E Freet Kim K Barthold Stephen W SW
Clinical and diagnostic laboratory immunology 20050401 4
Several species of helicobacter have been isolated from laboratory mice, including H. bilis, H. hepaticus, H. muridarum, H. rodentium, and H. typhlonius, which appear to be the most common. The most widely used published method for molecular detection of these agents is PCR amplification of a conserved region of 16S rRNA, but differential speciation requires restriction enzyme digestion of the amplicons. This study was undertaken to determine PCR conditions that would simultaneously and specific ...[more]