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3D printed bioabsorbable composite scaffolds of poly (lactic acid)-tricalcium phosphate-ceria with osteogenic property for bone regeneration.

ABSTRACT: The fabrication of customized implants by additive manufacturing has allowed continued development of the personalized medicine field. Herein, a 3D-printed bioabsorbable poly (lactic acid) (PLA)- β-tricalcium phosphate (TCP) (10 wt %) composite has been modified with CeO₂ nanoparticles (CeNPs) (1, 5 and 10 wt %) for bone repair. The filaments were prepared by melt extrusion and used to print porous scaffolds. The nanocomposite scaffolds possessed precise structure with fine print resolution, a homogenous distribution of TCP and CeNP components, and mechanical properties appropriate for bone tissue engineering applications. Cell proliferation assays using osteoblast cultures confirmed the cytocompatibility of the composites. In addition, the presence of CeNPs enhanced the proliferation and differentiation of mesenchymal stem cells; thereby, increasing alkaline phosphatase (ALP) activity, calcium deposition and bone-related gene expression. Results from this study have shown that the 3D printed PLA-TCP-10%CeO₂ composite scaffold could be used as an alternative polymeric implant for bone tissue engineering applications: avoiding additional/revision surgeries and accelerating the regenerative process.

SUBMITTER: Harb SV

PROVIDER: S-EPMC10776431 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature

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3D printed bioabsorbable composite scaffolds of poly (lactic acid)-tricalcium phosphate-ceria with osteogenic property for bone regeneration.

Harb Samarah V SV Kolanthai Elayaraja E Pugazhendhi Abinaya S AS Beatrice Cesar A G CAG Pinto Leonardo A LA Neal Craig J CJ Backes Eduardo H EH Nunes Ana C C ACC Selistre-de-Araújo Heloisa S HS Costa Lidiane C LC Coathup Melanie J MJ Seal Sudipta S Pessan Luiz A LA

Biomaterials and biosystems 20231218

The fabrication of customized implants by additive manufacturing has allowed continued development of the personalized medicine field. Herein, a 3D-printed bioabsorbable poly (lactic acid) (PLA)- β-tricalcium phosphate (TCP) (10 wt %) composite has been modified with CeO<sub>2</sub> nanoparticles (CeNPs) (1, 5 and 10 wt %) for bone repair. The filaments were prepared by melt extrusion and used to print porous scaffolds. The nanocomposite scaffolds possessed precise structure with fine print reso ...[more]

PMID: 38213985

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Project description:Additive manufacturing (AM), or 3D printing, of bioceramic scaffolds promises personalized treatment options for patients with site-specific designability for repair and reconstruction of bone defects. Although the theory for creating these complex geometries has already been made possible through AM's advancement, such shapes' manufacturability is difficult due to printing with ceramics' inherent complexities. Ceramics have the added challenge of being highly brittle, poor handleability of green (pre-sintered) parts, making complex shape high strength parts challenging to manufacture. This has led to a significant literature gap regarding the feasibility of creating bioceramic scaffolds with unique architectures that can be used in site-specific, individualized patient treatment. This work aims to successfully create complex topographical surfaces of cylindrical bone-like scaffolds to understand the correlation of increasing the scaffold surface area on mechanical properties and in vitro osteoblast cell proliferation. An increase in osteoblast cell proliferation and facilitation in cellular attachment can ultimately lead to improved bone healing. This work explores the printing parameters within an Innovent+® ExOne binder jet 3D printer to produce scaffold designs from synthesized tricalcium phosphate powder. Mechanical testing reveals the designed structures enhance scaffold compressive strength by 30% compared to control dense cylindrical scaffolds. Osteoblast cell proliferation is also increased due to changes in surface topography with a nearly 2-fold increase. Our work incorporates macro-level topographical changes to increase surface area, which is another avenue that could be combined with other scaffold features such as porosity. Results show bulk surface topography modifications via 3D printing can increase surface area to support enhanced biological response without compromising mechanical properties. This discovery may enable a future generation of porous scaffolds with external structures for further progress towards proper defect-specific synthetic bone grafts.

Dataset Information

3D printed bioabsorbable composite scaffolds of poly (lactic acid)-tricalcium phosphate-ceria with osteogenic property for bone regeneration.

Publications

3D printed bioabsorbable composite scaffolds of poly (lactic acid)-tricalcium phosphate-ceria with osteogenic property for bone regeneration.

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