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Phosphatidylinositol-phospholipase C4 suppresses the hypersensitive response of Nicotiana benthamiana.

ABSTRACT: Phospholipid signaling plays an important role in plant immune responses. Here, we isolated two phospholipase C4 (PLC4) orthologs in the Nicotiana benthamiana genome, designated as N. benthamiana PLC4-1 and PLC4-2 (NbPLC4-1 and NbPLC4-2). We created NbPLC4-1- and NbPLC4-2- silenced plants. Induction of the hypersensitive response (HR), including HR cell death and bacterial population reduction, was accelerated in both NbPLC4-1- and NbPLC4-2-silenced plants challenged with N. benthamiana-incompatible Ralstonia solanacearum 8107. The NbPLC4-1- and NbPLC4-2-silenced plants also showed enhanced expression of Nbhin1, a HR marker gene. Expressions of genes for salicylic acid (SA) and jasmonic acid (JA) signaling were drastically increased in NbPLC4-1- and NbPLC4-2-silenced plants by R. solanacearum inoculation. In addition, NbPLC4-1 and NbPLC4-2 silencing triggered reactive oxygen species (ROS) hyper-production. These results suggest that NbPLC4s are closely associated with JA, SA, and ROS signaling and act as negative regulators of the HR in N. benthamiana.

SUBMITTER: Fukui K 

PROVIDER: S-EPMC10777131 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature

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Phosphatidylinositol-phospholipase C4 suppresses the hypersensitive response of <i>Nicotiana benthamiana</i>.

Fukui Kotoko K   Ohnishi Kouhei K   Hikichi Yasufumi Y   Kiba Akinori A  

Plant biotechnology (Tokyo, Japan) 20230301 1

Phospholipid signaling plays an important role in plant immune responses. Here, we isolated two phospholipase C4 (PLC4) orthologs in the <i>Nicotiana benthamiana</i> genome, designated as <i>N. benthamiana PLC4-1</i> and <i>PLC4-2</i> (<i>NbPLC4-1</i> and <i>NbPLC4-2</i>). We created <i>NbPLC4-1-</i> and <i>NbPLC4-2</i>- silenced plants. Induction of the hypersensitive response (HR), including HR cell death and bacterial population reduction, was accelerated in both <i>NbPLC4-1-</i> and <i>NbPLC  ...[more]

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