Sucrose-phosphate synthase from Synechocystis sp. strain PCC 6803: identification of the spsA gene and characterization of the enzyme expressed in Escherichia coli.
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SUBMITTER: Curatti L
PROVIDER: S-EPMC107790 | biostudies-literature | 1998 Dec
REPOSITORIES: biostudies-literature
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Sucrose-phosphate synthase from Synechocystis sp. strain PCC 6803: identification of the spsA gene and characterization of the enzyme expressed in Escherichia coli.
Curatti L L Folco E E Desplats P P Abratti G G Limones V V Herrera-Estrella L L Salerno G G
Journal of bacteriology 19981201 24
The first identification and characterization of a prokaryotic gene (spsA) encoding sucrose-phosphate synthase (SPS) is reported for Synechocystis sp. strain PCC 6803, a unicellular non-nitrogen-fixing cyanobacterium. Comparisons of the deduced amino acid sequence and some relevant biochemical properties of the enzyme with those of plant SPSs revealed important differences in the N-terminal and UDP-glucose binding site regions, substrate specificities, molecular masses, subunit compositions, and ...[more]
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