Unknown

Dataset Information

0

The developmentally regulated alb1 gene of Aspergillus fumigatus: its role in modulation of conidial morphology and virulence.


ABSTRACT: Aspergillus fumigatus, an important opportunistic pathogen which commonly affects neutropenic patients, produces conidia with a bluish-green color. We identified a gene, alb1, which is required for conidial pigmentation. The alb1 gene encodes a putative polyketide synthase, and disruption of alb1 resulted in an albino conidial phenotype. Expression of alb1 is developmentally regulated, and the 7-kb transcript is detected only during the conidiation stage. The alb1 mutation was found to block 1,3,6,8-tetrahydroxynaphthalene production, indicating that alb1 is involved in dihydroxynaphthalene-melanin biosynthesis. Scanning electron microscopy studies showed that the alb1 disruptant exhibited a smooth conidial surface, whereas complementation of the alb1 deletion restored the echinulate wild-type surface. Disruption of alb1 resulted in a significant increase in C3 binding on conidial surfaces, and the conidia of the alb1 disruptant were ingested by human neutrophils at a higher rate than were those of the wild type. The alb1-complemented strain producing bluish-green conidia exhibited inefficient C3 binding and neutrophil-mediated phagocytosis quantitatively similar to those of the wild type. Importantly, the alb1 disruptant had a statistically significant loss of virulence compared to the wild-type and alb1-complemented strains in a murine model. These results suggest that disruption of alb1 causes pleiotropic effects on conidial morphology and fungal virulence.

SUBMITTER: Tsai HF 

PROVIDER: S-EPMC107801 | biostudies-literature | 1998 Jun

REPOSITORIES: biostudies-literature

altmetric image

Publications

The developmentally regulated alb1 gene of Aspergillus fumigatus: its role in modulation of conidial morphology and virulence.

Tsai H F HF   Chang Y C YC   Washburn R G RG   Wheeler M H MH   Kwon-Chung K J KJ  

Journal of bacteriology 19980601 12


Aspergillus fumigatus, an important opportunistic pathogen which commonly affects neutropenic patients, produces conidia with a bluish-green color. We identified a gene, alb1, which is required for conidial pigmentation. The alb1 gene encodes a putative polyketide synthase, and disruption of alb1 resulted in an albino conidial phenotype. Expression of alb1 is developmentally regulated, and the 7-kb transcript is detected only during the conidiation stage. The alb1 mutation was found to block 1,3  ...[more]

Similar Datasets

| S-EPMC103784 | biostudies-literature
| S-EPMC150101 | biostudies-literature
| S-EPMC3065306 | biostudies-literature
| S-EPMC7389560 | biostudies-literature
| S-EPMC6177592 | biostudies-literature
2021-09-08 | PXD008503 | Pride
| S-EPMC2950423 | biostudies-literature
| S-EPMC2446674 | biostudies-literature
| S-EPMC10890407 | biostudies-literature
| S-EPMC3255654 | biostudies-literature