Dataset Information
Purine and carbohydrate availability drive Enterococcus faecalis fitness during wound and urinary tract infections.
Ontology highlight
ABSTRACT: Importance
Although E. faecalis is a common wound pathogen, its pathogenic mechanisms during wound infection are unexplored. Here, combining a mouse wound infection model with in vivo transposon and RNA sequencing approaches, we identified the E. faecalis purine biosynthetic pathway and galactose/mannose MptABCD phosphotransferase system as essential for E. faecalis acute replication and persistence during wound infection, respectively. The essentiality of purine biosynthesis and the MptABCD PTS is driven by the consumption of purine metabolites by E. faecalis during acute replication and changing carbohydrate availability during the course of wound infection. Overall, our findings reveal the importance of the wound microenvironment in E. faecalis wound pathogenesis and how these metabolic pathways can be targeted to better control wound infections.
SUBMITTER: Tan CAZ
PROVIDER: S-EPMC10790769 | biostudies-literature | 2024 Jan
REPOSITORIES: biostudies-literature
Publications
Purine and carbohydrate availability drive <i>Enterococcus faecalis</i> fitness during wound and urinary tract infections.
mBio 20231211 1
<h4>Importance</h4>Although <i>E. faecalis</i> is a common wound pathogen, its pathogenic mechanisms during wound infection are unexplored. Here, combining a mouse wound infection model with <i>in vivo</i> transposon and RNA sequencing approaches, we identified the <i>E. faecalis</i> purine biosynthetic pathway and galactose/mannose MptABCD phosphotransferase system as essential for <i>E. faecalis</i> acute replication and persistence during wound infection, respectively. The essentiality of pur ...[more]
