Identification and characterization of the PutP proline permease that contributes to in vivo survival of Staphylococcus aureus in animal models.

ABSTRACT: Staphylococcus aureus is an important pathogen of humans and other animals, causing bacteremia, abscesses, endocarditis, and other infectious syndromes. A signature-tagged mutagenesis (STM) system was adapted for use in studying the genes required for in vivo survival of S. aureus. An STM library was ultimately created in S. aureus RN6390, with Tn917 being used to create the transposon mutations. Pools of S. aureus RN6390 mutants were screened in mouse abscess, bacteremia, and wound infection models for growth attenuation after in vivo passage. One of the mutants that was identified displayed marked attenuation following large-pool screening in all three animal models, which was confirmed in bacteremia and endocarditis models of infection with a smaller pool of mutants. Sequence analysis o

SUBMITTER: Schwan WR 

PROVIDER: S-EPMC107942 | biostudies-literature | 1998 Feb

REPOSITORIES: biostudies-literature