Use of single nucleotide polymorphisms in the plcR gene for specific identification of Bacillus anthracis.
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ABSTRACT:
SUBMITTER: Easterday WR
PROVIDER: S-EPMC1081367 | biostudies-literature | 2005 Apr
REPOSITORIES: biostudies-literature
Publications
Use of single nucleotide polymorphisms in the plcR gene for specific identification of Bacillus anthracis.
Easterday W Ryan WR Van Ert Matthew N MN Simonson Tatum S TS Wagner David M DM Kenefic Leo J LJ Allender Christopher J CJ Keim Paul P
Journal of clinical microbiology 20050401 4
A TaqMan-minor groove binding assay designed around a nonsense mutation in the plcR gene was used to genotype Bacillus anthracis, B. cereus, and B. thuringiensis isolates. The assay differentiated B. anthracis from these genetic near-neighbors and determined that the nonsense mutation is ubiquitous across 89 globally and genetically diverse B. anthracis strains. ...[more]
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