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Specific binding of G-quadruplex in SARS-CoV-2 RNA by RHAU peptide.

ABSTRACT: G-quadruplexes (G4s) are reported to present on the SARS-CoV-2 RNA genome and control various viral activities. Specific ligands targeting those viral nucleic acid structures could be investigated as promising detection methods or antiviral reagents to suppress this menacing virus. Herein, we demonstrate the binding between a G4 structure in the RNA of SARS-CoV-2 and a fluorescent probe created by fusing a parallel-G4 specific RHAU53 and a cyan fluorescent protein. The specific binding of G4 in SARS-CoV-2 by RHAU peptide was easily detected under the fluorescence spectrometer. The drawbacks of this approach and potential solutions are also discussed.

SUBMITTER: Nguyen LTA

PROVIDER: S-EPMC10824680 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature

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Specific binding of G-quadruplex in SARS-CoV-2 RNA by RHAU peptide.

Nguyen Le Tuan Anh LTA Nguyen Thao Thu Thi TTT Dang Dung Thanh DT

Current research in structural biology 20240105

G-quadruplexes (G4s) are reported to present on the SARS-CoV-2 RNA genome and control various viral activities. Specific ligands targeting those viral nucleic acid structures could be investigated as promising detection methods or antiviral reagents to suppress this menacing virus. Herein, we demonstrate the binding between a G4 structure in the RNA of SARS-CoV-2 and a fluorescent probe created by fusing a parallel-G4 specific RHAU53 and a cyan fluorescent protein. The specific binding of G4 in ...[more]

PMID: 38292819

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