Comparative analysis of bacterial diversity in freshwater sediment of a shallow eutrophic lake by molecular and improved cultivation-based techniques.
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ABSTRACT:
SUBMITTER: Tamaki H
PROVIDER: S-EPMC1082574 | biostudies-literature | 2005 Apr
REPOSITORIES: biostudies-literature
Publications
Comparative analysis of bacterial diversity in freshwater sediment of a shallow eutrophic lake by molecular and improved cultivation-based techniques.
Tamaki Hideyuki H Sekiguchi Yuji Y Hanada Satoshi S Nakamura Kazunori K Nomura Nakao N Matsumura Masatoshi M Kamagata Yoichi Y
Applied and environmental microbiology 20050401 4
Comparative analysis of bacterial diversity in freshwater sediment collected from a shallow eutrophic lake was performed by using 16S rRNA gene clone library and improved cultivation-based techniques. Our study demonstrated that the use of gellan gum as a gelling reagent instead of agar was more effective at increasing culturability, cultivating a diverse array of novel microbes, and reducing the gaps of the results between molecular and cultivation-based analyses. ...[more]
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