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Engineering of Bacillus thuringiensis Cry2Ab toxin for improved insecticidal activity.


ABSTRACT: Bacillus thuringiensis Cry2Ab toxin was a widely used bioinsecticide to control lepidopteran pests all over the world. In the present study, engineering of Bacillus thuringiensis Cry2Ab toxin was performed for improved insecticidal activity using site-specific saturation mutation. Variants L183I were screened with lower LC50 (0.129 µg/cm2) against P. xylostella when compared to wild-type Cry2Ab (0.267 µg/cm2). To investigate the molecular mechanism behind the enhanced activity of variant L183I, the activation, oligomerization and pore-formation activities of L183I were evaluated, using wild-type Cry2Ab as a control. The results demonstrated that the proteolytic activation of L183I was the same as that of wild-type Cry2Ab. However, variant L183I displayed higher oligomerization and pore-formation activities, which was consistence with its increased insecticidal activity. The current study demonstrated that the insecticidal activity of Cry2Ab toxin could be assessed using oligomerization and pore-formation activities, and the screened variant L183I with improved activity might contribute to Cry2Ab toxin's future application.

SUBMITTER: Fu BW 

PROVIDER: S-EPMC10834393 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature

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Engineering of Bacillus thuringiensis Cry2Ab toxin for improved insecticidal activity.

Fu Bai-Wen BW   Xu Lian L   Zheng Mei-Xia MX   Shi Yan Y   Zhu Yu-Jing YJ  

AMB Express 20240201 1


Bacillus thuringiensis Cry2Ab toxin was a widely used bioinsecticide to control lepidopteran pests all over the world. In the present study, engineering of Bacillus thuringiensis Cry2Ab toxin was performed for improved insecticidal activity using site-specific saturation mutation. Variants L183I were screened with lower LC<sub>50</sub> (0.129 µg/cm<sup>2</sup>) against P. xylostella when compared to wild-type Cry2Ab (0.267 µg/cm<sup>2</sup>). To investigate the molecular mechanism behind the enh  ...[more]

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