Ontology highlight
ABSTRACT:
SUBMITTER: Fu BW
PROVIDER: S-EPMC10834393 | biostudies-literature | 2024 Feb
REPOSITORIES: biostudies-literature
Fu Bai-Wen BW Xu Lian L Zheng Mei-Xia MX Shi Yan Y Zhu Yu-Jing YJ
AMB Express 20240201 1
Bacillus thuringiensis Cry2Ab toxin was a widely used bioinsecticide to control lepidopteran pests all over the world. In the present study, engineering of Bacillus thuringiensis Cry2Ab toxin was performed for improved insecticidal activity using site-specific saturation mutation. Variants L183I were screened with lower LC<sub>50</sub> (0.129 µg/cm<sup>2</sup>) against P. xylostella when compared to wild-type Cry2Ab (0.267 µg/cm<sup>2</sup>). To investigate the molecular mechanism behind the enh ...[more]